Journal: bioRxiv

Article Title: Modeling Human Peripheral Myelin Fully from Pluripotent Stem Cells and Immune-mediated Neuropathy In Vitro using ZIKA Virus

doi: 10.1101/2020.05.23.112748

Figure Lengend Snippet: a. Schematic summary of generation and isolation of EGFR + Schwann cells from human ES/iPS cells. b. Flow cytometric representative plots of EGFR + cells after 9 weeks of PNS differentiation protocol. c. Phase contrast image representative EGFR + cells morphology in culture (Scale bar, 500 μm) d. Representative immunofluorescence images of EGFR + cells for S100B, GFAP, and MPZ (Scale bar, 50 μm). e. Hierarchical clustering of whole-transcriptome expression profiles demonstrate wave of gene expression changes during differentiation from OCT4::EGFP (undifferentiated hPSCs), SOX10::EGFP (hPSC-NC) to EGFR positive cells, ( n = 3 biological replicates per groups) f. Heat map of the expression levels of selected Schwann cell related genes, ( n = 3 biological replicates per groups). g. Representative images for MBP and EGFP colocalization in the EGFR + EGFP + cells and PNS neurons co-culture (Scale bar, 50 μm). h. Representative confocal microscope images for NF, S100 and EGFP in P4 mice pup sciatic nerve sections of EGFR + EGFP + cells transplanted and contralateral control sides (Scale bar, 50 μm).

Article Snippet: Sciatic nerve microsurgery was carried out according to protocol , 1ul of (50,000 cells/ul) EGFP + EGFR + cells were injected inside the sciatic nerve of each neonatal CD1 mice (P4) (Charles River).

Techniques: Isolation, Immunofluorescence, Expressing, Co-Culture Assay, Microscopy